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        褚金芳
        联系电话:64806466
        E-Mail: jfchu@genetics.ac.cn
        办公地点:动物所D座S129
 
褚金芳,博士,高级工程师,硕士生导师,植物激素平台首席技术专家
 
        1998年毕业于中国海洋大学获学士学位,2001年获中国海洋大学硕士学位,2004年获中国科学院化学研究所博士学位。2005年初进入美国 Research Foundation of City University of New York (CUNY)从事博士后研究工作。2006年底回国工作,进入中科院遗传发育所,国家植物基因研究中心(北京),主要负责高灵敏度植物激素分析平台的建设与技术服务工作。
 
        研究方向为以植物激素及其代谢产物为研究对象,利用高灵敏度、高质量精度的色谱—质谱联用技术,进行植物激素及其相关代谢产物分析鉴定技术研究,为植物研究及现代农业发展提供技术支持。研究内容包括建立和发展植物激素及其代谢产物分析的新技术、新方法;开发合成植物样品中预处理及分离的新材料;开展重要生物过程和生理过程中植物激素动态变化分析及其在植物体内代谢变化的研究;筛选和鉴定未知植物激素相关化合物;建立植物激素分析平台。
 
主持科研项目(课题)及人才计划项目情况
        1. 国家自然科学基金面上项目,基于硼亲和磁性纳米材料和LC-MS/MS植物材料中油菜素内酯的分离检测新方法研究,2015-2018,主持
        2. 国家自然科学基金重大研究计划集成项目,建立高通量、高灵敏度On-line SPE-LC-MS/MS独角金内酯定量分析方法的研究,2015年,主持
        3 中科院现有关键技术人才资助,2015-2017
        4. 国家自然科学基金重大研究计划集成项目,植物激素检测平台分析功能的完善与分析技术的提高,2012-20132,主持
        5. 中科院功能仪器开发项目,高灵敏度植物激素在线分析方法,2010-2012,主持
        6. 国家自然科学基金重大研究计培育项目,高灵敏度植物激素检测平台的建立和检测技术的优化,2009-2011,主持
 
研究文章
1. Wang, B., Chu, J., Yu, T., Xu, Q., Sun, X., Yuan, J., Xiong, G., Wang, G., Wang, Y. and Li, J. (2015). Tryptophan-independent auxin biosynthesis contributes to early embryogenesis in Arabidopsis. PNAS 112: 4821-4826. 共第一作者)
 
2. Sun, X., Ouyang, Y., Chu, J., Yan, J., Yu, Y., Li, X., Yang, J. and Yan, C. (2014). An in-advance stable isotope labeling strategy for relative analysis of multiple acidic plant hormones in sub-milligram Arabidopsis thaliana seedling and a single seed. J Chromatogra A 1338: 67-76.
 
3. 夏群, 辛培勇, 褚金芳*. (2016) 全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素. 分析化学44 (3): 409-415. (*通讯作者)
 
4. Xin, P., Yan, J., Fan, J., Chu, J.* and Yan, C.* (2013). A dual role of boronate affinity in high-sensitivity detection of vicinal diol brassinosteroids from sub-gram plant tissues via UPLC-MS/MS. Analyst 138: 1342-1345.
 
5. Xin, P., Yan, J., Fan, J., Chu, J.* and Yan, C.* (2013). An improved simplified high-sensitivity quantification method for determining brassinosteroids in different tissues of rice and Arabidopsis. Plant Physiol 162: 2056-2066.
 
6. 欧阳玥, 孙晓红, 闫静, 褚金芳*, 闫存玉*. (2013). 稳定同位素标记技术在生物分子相对定量分析研究中的应用与进展. 科学通报 27: 2762-2778.
 
7. Fu, J., Sun, X., Wang, J., Chu, J.* and Yan, C.* (2011) Progress in quantitative analysis of Plant Hormones. Chinese Science Bulletin, 56(4-5): 355-366.
 
8. 符继红, 孙晓红, 王吉德, 褚金芳*, 闫存玉*. (2010) 植物激素定量分析方法研究进展. 科学通报 55: 3163-3176.
 
9. Fu, J., Chu, J., Sun, X., Wang, J. and Yan, C. (2012). Simple, rapid, and simultaneous assay of multiple carboxyl containing phytohormones in wounded tomatoes by UPLC-MS/MS using single SPE purification and isotope dilution. Anal Sci. 28 (11):1081-1087.
 
技术合作文章
1. Yao, R., Ming, Z., Yan, L., Li, S., Wang, F., Ma, S., Yu, C., Yang, M., Chen, L., Li, Y., Yan, C., Miao, D., Sun, Z., Yan, J., Sun, Y., Wang, L., Chu, J., Fan, S., He, W., Deng, H., Nan, F., Li, J., Rao, Z., Lou, Z. and Xie, D. (2016). DWARF14 is a non-canonical hormone receptor for strigolactone. Nature doi:10.1038/nature19073.
 
2. Ma, D., Li, X., Guo, Y., Chu, J., Fang S., Yan, C., Noel, J. P. and Liu, H. (2015). Cryptochrome 1 interacts with PIF4 to regulate high temperature-mediated hypocotyl elongation in response to blue light. PNAS 113: 225-229.
 
3. Zhang, X., Wu, Q., Cui, S., Ren, J., Qian, W., Yang, Y., He, S., Chu, J., Sun, X., Yan, C., Yu, X. and An, C. (2015). Hijacking of the jasmonate pathway by the mycotoxin fumonisin B1 (FB1) to initiate programmed cell death in Arabidopsis is modulated by RGLG3 and RGLG4. J Exp Bot 66: 2709-2721.
 
4. Yin, C., Ma, B., Collinge, D.P., Pogson, B.J., He, S.,  Xiong, Q.,  Duan, K., Chen, H., Yang, C., Lu, X., Wang, Y., Zhang, W., Chu, C., Sun, X., Fang, S., Chu, J., Lu, T., Chen, S. and Zhang, J. (2015). Ethylene responses in rice roots and coleoptiles are differentially regulated by a carotenoid isomerase-mediated abscisic acid pathway. Plant Cell 27: 1061-1081.
 
5. Xi, L., Wen, C., Fang, S., Chen, X., Nie, J., Chu, J., Yuan, C., Yan, C., Ma, N., Zhao, L. (2015). Impacts of strigolactone on shoot branching under phosphate starvation in chrysanthemum (Dendranthema grandiflorum cv. Jinba). Front Plant Sci 6. DOI: 10.3389/fpls.2015.00694
 
6. Liu, L., Tong, H., Xiao, Y., Che, R., Xu, F., Hu, B., Liang, C., Chu, J., Li, J., Chu, C. (2015). Activation of Big Grain1 significantly improves grain size by regulating auxin transport in rice. PNAS 112: 11102-11107.
 
7. Ma, X., Ma, J., Zhai, H., Xin P., Chu, J., Qiao Y., Han, L. (2015). CHR729 Is a CHD3 Protein That Controls Seedling Development in Rice. Plos One 10.
 
8. Xu, F., Fang, J., Ou, S., Gao, S., Zhang, F., Du, L., Xiao, Y., Wang, H., Sun, X., Chu, J., Wang, G. and Chu, C. (2015). Variations in CYP78A13 coding region influence grain size and yield in rice. Plant Cell Environ 38: 800-811.
 
9. Sang, D., Chen, D., Liu, G., Liang, Y., Huang, L., Meng, X., Chu, J., Sun, X., Dong, G., Yuan, Y., Qian, Q., Li, J. and Wang, Y. (2014). Strigolactones regulate rice tiller angle by attenuating shoot gravitropism through inhibiting auxin biosynthesis. PNAS 111: 11199-11204.
 
10. Liang, C., Wang, Y., Zhu, Y., Tang, J., Hu, B., Liu, L., Ou, S., Wu, H., Sun, X., Chu, J. and Chu, C. (2014). OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice. PNAS 111: 10013-10018.
 
11. Gao, S., Fang, J., Xu, F., Wang, W., Sun, X., Chu, J., Cai, B., Feng, Y. and Chu, C. (2014). CYTOKININ OXIDASE/DEHYDROGENASE4 integrates cytokinin and auxin signaling to control rice crown root formation. Plant Physiol 165: 1035-1046.
 
12. Zhang, Y., Yu, Y., Wang, C., Li, Z., Liu, Q., Xu, J., Liao, J., Wang, X., Qu, L., Chen, F., Xin, P., Yan, C., Chu, J., Li, H. and Chen, Y. (2013). Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching. Nature Biotechnol 31: 848-852.
 
13. Zhou, F., Lin, Q., Zhu, L., Ren, Y., Zhou, K., Shabek, N., Wu, F., Mao, H., Dong, W., Gan, L., Ma, W., Gao, H., Chen, J., Yang, C., Wang, D., Tan, J., Zhang, X., Guo, X., Wang, J., Jiang, L., Liu, X., Chen, W., Chu, J., Yan, C., Ueno, K., Ito, S., Asami, T., Cheng, Z., Wang, J., Lei, C., Zhai, H., Wu, C., Wang, H., Zheng, N. and Wan, J. (2013). D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling. Nature 504: 406.
 
14. Qi, L., Yan, J., Li, Y., Jiang, H., Sun, J., Chen, Q., Li, H., Chu, J., Yan, C., Sun, X., Yu, Y., Li, C. and Li, C. (2012). Arabidopsis thaliana plants differentially modulate auxin biosynthesis and transport during defense responses to the necrotrophic pathogen alternaria brassicicola. New Phytol 195: 872-882.
 
15. Sun, J., Qi, L., Li, Y., Chu, J. and Li, C. (2012). PIF4-Mediated Activation of YUCCA8 Expression Integrates Temperature into the Auxin Pathway in Regulating Arabidopsis Hypocotyl Growth. Plos Genet 8.
 
16. Wu, H., Zhang, Z., Wang, J., Oh, D., Dassanayake, M., Liu, B., Huang, Q., Sun, H., Xia, R., Wu, Y., Wang, Y., Yang, Z., Liu, Y., Zhang, W., Zhang, H., Chu, J., Yan, C., Fang, S., Zhang, J., Wang, Y., Zhang, F., Wang, G., Lee, S. Y., Cheeseman, J. M., Yang, B., Li, B., Min, J., Yang, L., Wang, J., Chu, C., Chen, S., Bohnert, H. , Zhu, J., Wang, X. and Xie, Q. (2012). Insights into salt tolerance from the genome of Thellungiella salsuginea. PNAS 109: 12219-12224.
 
17. He, W., Brumos, J., Li, H., Ji, Y., Ke, M., Gong, X., Zeng, Q., Li, W., Zhang, X., An, F., Wen, X., Li, P., Chu, J., Sun, X., Yan, C., Yan, N., Xie, D., Raikhel, N., Yang, Z., Stepanova, A. N., Alonso, J. M. and Guo, H. (2011). A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis. Plant Cell 23: 3944-3960.
 
18. Cui, H., Wang, Y., Xue, L., Chu, J., Yan, C., Fu, J., Chen, M., Innes, R. W. and Zhou, J. (2010). Pseudomonas syringae effector protein AvrB perturbs Arabidopsis hormone signaling by activating MAP kinase 4. Cell Host & Microbe 7: 164-175.
 
19. Zhou, W., Wei, L., Xu, J., Zhai, Q., Jiang, H., Chen, R., Chen, Q., Sun, J., Chu, J., Zhu, L., Liu, C.-M. and Li, C. (2010). Arabidopsis tyrosylprotein sulfotransferase acts in the auxin/PLETHORA pathway in regulating postembryonic maintenance of the root stem cell niche. Plant Cell 22: 3692-3709.
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